Why the need for qPCR publication guidelines? – The case for MIQE

Abstract

MIQE (the minimum information for the publication of quantitative real-time PCR experiments) was conceived as a set of guidelines to encourage more comprehensive communication of experimental detail within publications utilising qPCR technology. Although MIQE is beginning to have an impact on the quality of data published, some journals, frequently with high impact factors, continue to publish misleading and technically inadequate research papers. MIQE is not a pedantic set of instructions that makes publication a more arduous chore. Instead, MIQE’s extensive reference to and incorporation of the many variables that make up the pre-assay, assay and data analysis steps makes it an ideal template for novice and experienced user alike on which to design every future qPCR assay. A concerted effort to implement these guidelines is essential if qPCR is to mature into a robust, accurate and reliable quantification technology.