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Mikael Kubista 1, Amin Forootan2 1 TATAA Biocenter AB, Göteborg, Sweden 2 MultiD Analyses AB, Göteborg, Sweden |
Abstract
GenEx was the first commercial software developed for qPCR, and has since then become market leader with few thousands of users and several thousands of publications using GenEx. Sup- ported by all leading qPCR instrument manufacturers GenEx has batch import and corrects for inter-run variation. During pre- processing missing values are imputed, outliers are handled, and data are optimally normalized. Absolute quantification includes determining limit of detection, limit of quantification and dynamic range. All parameters are determined with confidence ranges in line with CLSI, CEN and ISO guidelines. Expression data are ana- lyzed with univariate methods including t-test, non-parametric methods and various ANOVAs with appropriate corrections for multiple testing ambiguity or multivariate methods including hierarchical clustering, Principal Component Analysis (PCA), Self- Organizing Map (SOM), Artificial Neural Network (ANN), Support Vector Machines (SVM), and many more. All analyses are easily per- formed with few clicks with the mouse using intuitive workflows that generate results that are readily interpreted in a biologi- cal context. At this lunch seminar, we will present GenEx and exemplify how easily multiple run files are in imported, measured data pre-processed, and how data are interactively analyzed using dynamic PCA to reveal key expression pathways and correlations across samples. All participants will receive FREE GenEx license for limited time.
http://dx.doi.org/10.1016/j.bdq.2017.02.052
Download a free GenEx trial version on GenEx.gene-quantification.info
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. SA Bustin et al., Clinical Chemistry 55 (4), 611-622
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