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Svilen Tzonev Bio-Rad, United States of America |
Abstract
Advances in digital PCR and Next Gen Sequencing technologies continue to expand the use of digital approaches in Biology. Both techniques produce large amounts of digital, high definition data and are natural complements to each other. This presentation will discuss droplet digital PCR technologies and applications developed by Bio-Rad and its customers. Some examples include absolute DNA quantitation, copy number analysis, rare mutation and rare species detection, gene expression down to the single cell. Emerging applications include viral load detection, GMO load, carrier status, phasing enzymatic activity and many others. We will also discuss application of ddPCR for NGS library quantitation and quality control and novel approaches for sample prep in droplets for sequencing.
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