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Radek Sindelka, Pavel Abaffy, Ravindra Naraine, Mikael Kubista Institute of Biotechnology, Czech Republic |
Abstract
Starting from a single fertilized egg and then followed by various divisional steps, a complex organism is developed that has a distinct head-tail (bottom-up), left-right and dorsal-ventral (back-belly) asymmetrical axes. One of the main challenges in developmental biology is to understand how and when these asymmetries are generated and how they are controlled. Xenopus laevis (African clawed frog) and Acipenser ruthenus (sturgeon) are ideal models for studies of early development because of their large eggs and embryos. We have developed a unique molecular tomography platform based on RT-qPCR, RNA-seq and iTraq UPLC-ESI-MS/MS to measure asymmetric localization of fate determining materials such as mRNAs, non-coding RNAs and proteins within the early developmental stages. Additionally, we have developed new tools and approaches for analyzing data originating from spatial transcriptomics. Herein we present the results from our work.
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