Single Molecule RNA FISH: novel, simple, and accurate quantitative applications for gene expression analysis

Abstract
We have developed a simple, direct method for specifically detecting individual RNA molecules in situ via fluorescence microscopy. Our RNA-FISH method relies on using large numbers of fluorescently labeled oligonucleotides designed to target particular RNA species. This approach is highly specific and highly sensitive and provides absolute, direct quantification of RNA abundance in single cells. Moreover, it is applicable to a broad range of biological sample types, ranging from microbes to human tissue section. We will discuss the method itself as well as address some biological applications in development and cancer.