MIQE qPCR & dPCR
How to apply the MIQE guidelines – a visual, interactive and practical qPCR & dPCR guide
Free download of the 5th edition (published 20th Feb 2022)
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Follow the talk by Prof. Afif Abdel Nour introducing the 1st edition “MIQE & qPCR iBook – a digital publication: How making the MIQE guidelines easier to follow” |
MIQE and qPCR QC talks from GQ2023 – Freising
- Genex For the Analysis of QPCR And DPCR Data in Regulated Environments
- QScript Ultra RT: A Next Generation Reverse Transcription Platform to Break Cold-chain and Democratize State of the Art RNA Quantification Tools
- Guidance for the Validation of qPCR/dPCR Assays in Clinical Research
- One More Time: Do Not Ignore the Amplification Efficiency!
- Absolute Quantification of Estimated Copy Number Without a Standard Curve
- Assessing Nucleic Acid-based Therapeutics – Fully Integrated Laboratory Workflow for Regulated Bioanalysis
- Importance of Quality Control of In Vitro Transcribed RNA
MIQE and qPCR QC talks from GQ2019 – Freising
- Quality Standards in quantitative PCR; Specification, Validation, Controls and Standards
- Directed Evolution of Enzymes for Streamlined and Reliable RT-qPCR and NGS Workflows
- Multiplex Mediator Probe Real-Time PCR: Optimisation and Guideline Development through Systematic Characterisation of Label Free Mediator Probes and Fluorogenic Universal Reporters
MIQE and qPCR QC talks from GQ2017 – Freising
- Why are we still using qPCR for the quantification of nucleic acids?
- Target concentration and replicate qPCR reactions
- Laboratory automation and data management in diagnostics – qPCR ZIKV detection and quantification
- Validation of a qPCR method – Determining limit of detection, limit of quantification and dynamic range
- Influence of PCR consumables on the accuracy of real-time PCR experiments and NGS sample preparation
- How to monitor analytical and technical factors influencing qPCR
MIQE and qPCR QC talks from qPCR & NGS 2015 – Freising
- Variability of the Reverse Transcription Step: Practical Implications
- MIQE & qPCR iBook — a digital publication
- qScript XLT cDNA SuperMix: overcoming the common pitfalls of cDNA synthesis as applied to relative quantification and RT-qPCR
- New tools for quality assessment of RNA in Molecular Diagnostics
- ThermaStop™ Hot-Start Reagent: Properties and Benefits
- Engineered DNA Polymerases
- Reducing time and error rates by automating qPCR workflows
- Middleware Solutions To Improve Molecular Diagnostics; Connectivity, Quality Control And Lean Processes
MIQE and qPCR QC talks from qPCR & NGS 2013 – Freising
- MIQE 2009-2013 – its impact four years after publication
- Quality control in quantitative PCR
- Applying The MIQE guidelines to clinical and pre-clinical trials
- Applying the MIQE Guidelines to Screens Utilizing qPCR Focused Arrays
- Management and Automation of qPCR Diagnostic Workflows
- Four Years of RDML qPCR Data Format – Achievements and Opportunities
- The Impact of MIQE Guidelines in the Plant Science Community
MIQE and qPCR QC talks from qPCR 2011 – Freising
- MIQE: compliance and amendments
- Convenient and reliable gene expression profiling using RealTime ready Focus Panels in combination with sound data-analysis using qbasePLUS
- “Stay in touch while on the bench” MIQE qPCR
MIQE and qPCR QC talks from qPCR 2010 – Vienna
- Why the need for qPCR publication guidelines: The case for MIQE
- A Practical Approach to Assay Design for qPCR – Overcoming Difficult Assays, Designs and Optimizations while Conforming to the MIQE Guidelines
External MIQE talks
- Scientific Integrity: Can Molecular Medicine Be Trusted
- MIQE Guidelines: A Roadmap for Proper qPCR Experimental Design and Reporting
- Deliver Verifiable qPCR Results using MIQE Guidelines and Automation
- Following the MIQE Guidelines for RT-qPCR Experiments
- Application of MIQE Guidelines to qPCR
- qPCR and MIQE Seminar Series
As part of our customer education program, we have provided two recorded seminar series covering the topics of qPCR and MIQE. The recorded sessions are intended to provide a high level overview of these subject matters. We have kept the lessons concise so that you can enjoy a self-paced learning program.
MIQE publications
- English version (2009) The MIQE Guidelines – Minimum Information for Publication of Quantitative Real-Time PCR Experiments
- English version (2013) The dMIQE Guidelines – Guidelines for Minimum Information for Publication of Quantitative Digital PCR Experiments
- English version (2020) The Digital MIQE Guidelines Update – Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020
- Chinese version (2010) The MIQE Guidelines – Minimum Information for Publication of Quantitative Real-Time PCR Experiments
- Japanese version (2013) The MIQE Guidelines – Minimum Information for Publication of Quantitative Real-Time PCR Experiments
- Arabian version (2013) The MIQE Guidelines – Minimum Information for Publication of Quantitative Real-Time PCR Experiments
