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Ken Livak Fluidigm Corporation, United States of America |
Abstract
Fluctuations in gene expression at the single cell level could be key for generating developmental signals. Data needs to be collected from a statistically significant number of single cells in order to determine the range of gene expression present in a population of cells. Furthermore, transcripts need to be quantified for a number of genes in order to obtain meaningful cell signatures. Fluidigm’s BioMark arrays provide a convenient and cost-effective system for performing multiple RNA expression assays on multiple single-cell samples. The 96.96 array enables running up to 96 real time qPCR assays on up to 96 samples. This system has been used to study single cell gene expression in embryonic stem cells, cancer stem cells, and early stage embryos.
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