Automated PCR Setup for High Throughput Analysis Enabled by the Labcyte Echo® 525 Liquid Handler and Access™ Workstation

Celeste Glazer, Carl Jarman Labcyte, United States of America

Abstract

The Echo 525 liquid handler enables PCR assay setup for real-time quantitative PCR (qPCR), SNP analysis and DNA sequencing setup in reaction volumes from 10 µL to as little as 250 nL. The Echo liquid handler eliminates the risk of cross contamination and the requirement to pre-dilute samples or PCR reagents, enabling PCR setup and optimization in 384- and 1536-well microplates with greater efficiency. The Access workstation combines the Echo liquid handler with a range of devices (including systems for qPCR analysis) into flexible and modular solutions targeted for walk away, cost-effective high throughput PCR setup. This tutorial discusses validation of the Echo Liquid Handler for PCR setup and the implementation of an Access workstation for fully automated processing.