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Anna Elisabet Edlund 1, Shibu Yooseph 2 , Wenyuan Shi 3 , Xuesong He 3 , Jeffrey Scott McLean 4 1 J Craig Venter Institute, Genome Medicine Group, CA, USA 2 University of Central Florida, College of Engineering and Computer Science, FL, USA 3 University of California Los Angeles, Shool of Dentistry, CA, USA 4 University of Washington, School of Dentistry, WA, USA |
Abstract
Although oral microbial communities are subjected to daily physical and chemical disturbances such as fluctuations in pH, antimicrobial agents, dietary components and personal hygiene measures, a long-term stable microbiome persists. Biological pro- cesses that support this stability are important for the prevention of dysbiosis—a microbial shift toward a disease, e.g. periodontitis (gum disease) or caries (tooth decay), the two most common infec- tious diseases of man. In our previous work, by studying an oral in vitro biofilm model system greater than 100 bacterial species, we identified a plethora of metabolic activities possibly associated with oral health both at the gene and molecule level. We also showed that metabolic activities varied greatly for individual bacterial key-community members during pH fluctuations. Here, we focus on understanding molecular mechanisms and species interactions critical for biofilm community stability during a 24-hour incubation period. By applying meta-omics approaches we dissect regulatory pathways that control the plankton-to-biofilm transition and the maintenance of the stable oral biofilm community during pH stress. These approaches also allow us to target gene transcription activi- ties of virulence mechanisms in low pH over time.
http://dx.doi.org/10.1016/j.bdq.2017.02.054
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