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Alexander Rácz Eurofins MWG Operon, Germany |
Abstract
Over the past 20 years, quantitative polymerase chain reaction (qPCR) has become a powerful tool for nucleic acid quantification. Together with the development of a broad range of applications for gene expression or mutation analysis, there was a continuous evolution of instrument hardware, detection formats or strategies and consumables. Hybridisation probes like DualLabeled Probes (DLP´s), Molecular Beacons or FRET-Probes have several advantages over unspecific intercalation dyes. In this talk we describe the impact of dyes and quencher molecules on the performance of qPCR experiments and present the features and benefits of next generation dyes for qPCR.
| Back to MIQE and QC strategies in qPCR |
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