10 Years of Digital MIQE, What Have We Learned?

10 Years of Digital MIQE, What Have We Learned?

Abstract
Digital PCR (dPCR) offers a unique approach for molecular analysis as it counts DNA molecules and is capable of doing this with high accuracy and reproducibility. dPCR also offers a number of unique analytical opportunities. The method provides high quantitative precision and the fact that each DNA molecule has its own minute reaction (termed partition) means rare genetic variants are easier to detect. Yet for dPCR to be performed with the most success there are a number of considerations that are needed and, for an experiment to be independently evaluated, factors that should be reported. The Minimum Information for Publication of Quantitative Digital PCR Experiments (dMIQE), originally published in 2013, was updated in 2020 to reflect how the technology has matured. This talk will introduce dMIQE2020, which was supported by nine dPCR instrument manufacturers, explore how the technological applications have advanced and discuss what we have learnt in the last decade.